Biography

1982	Graduated from Chiba University, School of Medicine
1982-1986	Residency for Chest Medicine and ER at Chiba University Hospital
1986-1990	Graduate School for Immunology at Chiba University
1990	PhD in Immunology at Chiba University
1990-1997	Research Fellow of Molecular Pathology at Tokyo Metropolitan Institute of Gerontology (TMIG)
1997-2003	Laboratory Chief for Molecular Genetics of Gerontology at TMIG
2003-2007	Director of Molecular Genetics of Gerontology at TMIG
2004-present	Shirasawa Clinical Research Center
2007-2015	Professor, Juntendo University Medical Graduate School, Aging Control Medicine Dept.
2012-present	President, Shirasawa Anti-Aging Medical Institute
2013-present	Director, Japan Functional Diet Association
2013-present	Director, Japan Anti-Aging Food Association
2015	Adjunct professor University of Michigan, Medical School,Neurology
2015-present	Visiting Professor, University of Michigan, Neurology
2016.04	Specially appointed professor, Dokkyo Medical University Department of Physiology and Biological Information
2016-2017	Chief Adviser, Shinjuku Shirasawa Memorial Clinic
2017-	Director at Ochanomizu Longevity&Health Clinic

Research Topics and Interests

1. Molecular Biology of Aging and Gerontology
2. Molecular Pathology of Age-related Diseases
3. Molecular Genetics of Alzheimer's Diseases
4. Ketogenic Diet and Alzheimer's Disease
5. Ketone body: Physiology and Pathology
6. Nutrition and Preventive Medicine
7. Telomere research
8. Stem Cell research
9. Physical exercise and Aging Control
10. Analysis of Athletic Gene

Academic Activities

• Japan Anti-Aging Medical Association, Director
• Japan Biogerontology, Director
• International Biophilia Rehabilitation Academy, Director
• Japan Functional Diet Association, Director
• Japan Anti-Aging Food Association, Director

• Journal Editorial
• Journal of Neuroscience Research
• Journal of Anti-Aging Medicine
• Biophilia Rehabilitation Journal

Scientific Papers 2018 - 2016

2018

Yu, Y., T. Shintani, Y. Takeuchi, T. Shirasawa, and M. Noda, Protein Tyrosine Phosphatase Receptor Type J (PTPRJ) Regulates Retinal Axonal Projections by Inhibiting Eph and Abl Kinases in Mice. J Neurosci, 2018. 38(39): p. 8345-8363.

Takahashi, K., Y. Miura, I. Ohsawa, T. Shirasawa, and M. Takahashi, In vitro rejuvenation of brain mitochondria by the inhibition of actin polymerization. Sci Rep, 2018. 8(1): p. 15585.

2017

Takahashi, K., I. Ohsawa, T. Shirasawa, and M. Takahashi, Optic atrophy 1 mediates coenzyme Q-responsive regulation of respiratory complex IV activity in brain mitochondria. Exp Gerontol, 2017. 98: p. 217-223.

Naraoka, Y., H. Harada, M. Katagiri, H. Yamamura, and T. Shirasawa, N-acetyl glucosamine and proteoglycan containing supplement improves the locomotor functions of subjects with knee pain. Drug Discov Ther, 2017. 11(3): p. 140-145.

2016

Takahashi, K., I. Ohsawa, T. Shirasawa, and M. Takahashi, Early-onset motor impairment and increased accumulation of phosphorylated alpha-synuclein in the motor cortex of normal aging mice are ameliorated by coenzyme Q. Exp Gerontol, 2016. 81: p. 65-75.

Scientific Papers 2015 - 2011

2015

Tachibana, K., K. Sakurai, H. Yokoh, T. Ishibashi, K. Ishikawa, T. Shirasawa, and K. Yokote, Mutation in insulin receptor attenuates oxidative stress and apoptosis in pancreatic beta-cells induced by nutrition excess: reduced insulin signaling and ROS. Horm Metab Res, 2015. 47(3): p. 176-83.

Shibata, H., S. Hashimoto, K. Shimizu, R. Kawasato, T. Shirasawa, T. Yokota, H. Onoda, T. Okamoto, J. Nishikawa, N. Matsunaga, and I. Sakaida, Beneficial effects of combining computed tomography enteroclysis/enterography with capsule endoscopy for screening tumor lesions in the small intestine. Gastroenterol Res Pract, 2015. 2015: p. 952787.

Munetomo, A., Y. Hojo, S. Higo, A. Kato, K. Yoshida, T. Shirasawa, T. Shimizu, A. Barron, T. Kimoto, and S. Kawato, Aging-induced changes in sex-steroidogenic enzymes and sex-steroid receptors in the cortex, hypothalamus and cerebellum. J Physiol Sci, 2015. 65(3): p. 253-63.

Kobayashi, K., H. Nojiri, Y. Saita, D. Morikawa, Y. Ozawa, K. Watanabe, M. Koike, Y. Asou, T. Shirasawa, K. Yokote, K. Kaneko, and T. Shimizu, Mitochondrial superoxide in osteocytes perturbs canalicular networks in the setting of age-related osteoporosis. Sci Rep, 2015. 5: p. 9148.

2014

Takahashi, K., Y. Noda, I. Ohsawa, T. Shirasawa, and M. Takahashi, Extended lifespan, reduced body size and leg skeletal muscle mass, and decreased mitochondrial function in clk-1 transgenic mice. Exp Gerontol, 2014. 58: p. 146-53.

Sunagawa, T., T. Shimizu, A. Matsumoto, M. Tagashira, T. Kanda, T. Shirasawa, and H. Nakaya, Cardiac electrophysiological alterations in heart/muscle-specific manganese-superoxide dismutase-deficient mice: prevention by a dietary antioxidant polyphenol. Biomed Res Int, 2014. 2014: p. 704291.

Kojima, T., M. Dogru, O.M. Ibrahim, T. Nagata, K. Higa, T. Shimizu, T. Shirasawa, Y. Satake, S. Shimazaki, J. Shimazaki, and K. Tsubota, The effects of 3% diquafosol sodium application on the tear functions and ocular surface of the Cu,Zn-superoxide dismutase-1 (Sod1)-knockout mice. Mol Vis, 2014. 20: p. 929-38.

2013

Pokorski, M., M. Izumizaki, and T. Shirasawa, Chemosensory ventilatory responses in the mutant mice with Presbyterian hemoglobinopathy. Respir Physiol Neurobiol, 2013. 187(1): p. 18-25.

Ota, H., M. Akishita, H. Tani, T. Tatefuji, S. Ogawa, K. Iijima, M. Eto, T. Shirasawa, and Y. Ouchi, trans-Resveratrol in Gnetum gnemon protects against oxidative-stress-induced endothelial senescence. J Nat Prod, 2013. 76(7): p. 1242-7.

Ohguchi, T., T. Kojima, O.M. Ibrahim, T. Nagata, T. Shimizu, T. Shirasawa, T. Kawakita, Y. Satake, K. Tsubota, J. Shimazaki, and S. Ishida, The effects of 2% rebamipide ophthalmic solution on the tear functions and ocular surface of the superoxide dismutase-1 (sod1) knockout mice. Invest Ophthalmol Vis Sci, 2013. 54(12): p. 7793-802.

Koyama, H., H. Nojiri, S. Kawakami, T. Sunagawa, T. Shirasawa, and T. Shimizu, Antioxidants improve the phenotypes of dilated cardiomyopathy and muscle fatigue in mitochondrial superoxide dismutase-deficient mice. Molecules, 2013. 18(2): p. 1383-93.

Konno, H., Y. Kanai, M. Katagiri, T. Watanabe, A. Mori, T. Ikuta, H. Tani, S. Fukushima, T. Tatefuji, and T. Shirasawa, Melinjo (Gnetum gnemon L.) Seed Extract Decreases Serum Uric Acid Levels in Nonobese Japanese Males: A Randomized Controlled Study. Evid Based Complement Alternat Med, 2013. 2013: p. 589169.

2012

Takahashi, M., M. Ogawara, T. Shimizu, and T. Shirasawa, Restoration of the behavioral rates and lifespan in clk-1 mutant nematodes in response to exogenous coenzyme Q(10). Exp Gerontol, 2012.

Shirasawa, T., [Longevity society and bio-gerontology]. Nihon Ronen Igakkai Zasshi, 2012. 49(1): p. 36-9.

Noda, Y., K. Ota, T. Shirasawa, and T. Shimizu, Copper/zinc superoxide dismutase insufficiency impairs progesterone secretion and fertility in female mice. Biol Reprod, 2012. 86(1): p. 1-8.

Murakami, K., N. Murata, Y. Noda, K. Irie, T. Shirasawa, and T. Shimizu, Stimulation of the amyloidogenic pathway by cytoplasmic superoxide radicals in an Alzheimer's disease mouse model. Biosci Biotechnol Biochem, 2012. 76(6): p. 1098-103.

Miura, G., K. Kato, T. Shimizu, D. Shiga, and T. Shirasawa, Heme oxygenase-1 (HO-1) is constitutively up-regulated in top alpinists. Biochem Biophys Res Commun, 2012. 417(1): p. 104-8.

Lustgarten, M.S., A. Bhattacharya, F.L. Muller, Y.C. Jang, T. Shimizu, T. Shirasawa, A. Richardson, and H. Van Remmen, Complex I generated, mitochondrial matrix-directed superoxide is released from the mitochondria through voltage dependent anion channels. Biochem Biophys Res Commun, 2012. 422(3): p. 515-21.

Kojima, T., T.H. Wakamatsu, M. Dogru, Y. Ogawa, A. Igarashi, O.M. Ibrahim, T. Inaba, T. Shimizu, S. Noda, H. Obata, S. Nakamura, A. Wakamatsu, T. Shirasawa, J. Shimazaki, K. Negishi, and K. Tsubota, Age-related dysfunction of the lacrimal gland and oxidative stress: evidence from the Cu,Zn-superoxide dismutase-1 (Sod1) knockout mice. Am J Pathol, 2012. 180(5): p. 1879-96.

Katagiri, M., A. Satoh, S. Tsuji, and T. Shirasawa, Effects of astaxanthin-rich Haematococcus pluvialis extract on cognitive function: a randomised, double-blind, placebo-controlled study. J Clin Biochem Nutr, 2012. 51(2): p. 102-7.

Fujita, H., H. Fujishima, K. Takahashi, T. Sato, T. Shimizu, T. Morii, T. Shimizu, T. Shirasawa, Z. Qi, M.D. Breyer, R.C. Harris, Y. Yamada, and T. Takahashi, SOD1, but not SOD3, deficiency accelerates diabetic renal injury in C57BL/6-Ins2(Akita) diabetic mice. Metabolism, 2012. 61(12): p. 1714-24.

2011

Xue, Y.L., T. Ahiko, T. Miyakawa, H. Amino, F. Hu, K. Furihata, K. Kita, T. Shirasawa, Y. Sawano, and M. Tanokura, Isolation and Caenorhabditis elegans lifespan assay of flavonoids from onion. J Agric Food Chem, 2011. 59(11): p. 5927-34.

Toda, T., T. Sunagawa, T. Kanda, M. Tagashira, T. Shirasawa, and T. Shimizu, Apple Procyanidins Suppress Amyloid beta-Protein Aggregation. Biochem Res Int, 2011. 2011: p. 784698.

Sunagawa, T., T. Shimizu, T. Kanda, M. Tagashira, M. Sami, and T. Shirasawa, Procyanidins from apples (Malus pumila Mill.) extend the lifespan of Caenorhabditis elegans. Planta Med, 2011. 77(2): p. 122-7.

Shirasawa, T., Longevity genes and successful aging. Nihon Ronen Igakkai Zasshi, 2011. 48(2): p. 120-2.

Shimojo, Y., K. Kosaka, and T. Shirasawa, Effect of Ganoderma lucidum extract on adipocyte differentiation and adiponectin gene expression in the murine pre-adipocyte cell line, 3T3-L1. Phytother Res, 2011. 25(2): p. 202-7.

Shimizu, T. and T. Shirasawa, Biomarkers for aging. Nihon Yakurigaku Zasshi, 2011. 138(2): p. 60-3.

Shimizu, T., T. Baba, M. Ogawara, and T. Shirasawa, Lifespan and glucose metabolism in insulin receptor mutant mice. J Aging Res, 2011. 2011: p. 315640.

Sasaki, T., T. Shimizu, T. Koyama, M. Sakai, S. Uchiyama, S. Kawakami, Y. Noda, T. Shirasawa, and S. Kojima, Superoxide dismutase deficiency enhances superoxide levels in brain tissues during oxygenation and hypoxia-reoxygenation. J Neurosci Res, 2011. 89(4): p. 601-10.

Parajuli, N., A. Marine, S. Simmons, H. Saba, T. Mitchell, T. Shimizu, T. Shirasawa, and L.A. Macmillan-Crow, Generation and characterization of a novel kidney-specific manganese superoxide dismutase knockout mouse. Free Radic Biol Med, 2011. 51(2): p. 406-16.

Nojiri, H., Y. Saita, D. Morikawa, K. Kobayashi, C. Tsuda, T. Miyazaki, M. Saito, K. Marumo, I. Yonezawa, K. Kaneko, T. Shirasawa, and T. Shimizu, Cytoplasmic superoxide causes bone fragility owing to low-turnover osteoporosis and impaired collagen cross-linking. J Bone Miner Res, 2011. 26(11): p. 2682-94.

Noda, Y., K. Ota, T. Shirasawa, and T. Shimizu, Copper/Zinc-Superoxide Dismutase Insufficiency Impairs Progesterone Secretion and Fertility in Female Mice. Biol Reprod, 2011.

Murakami, K., S. Yokoyama, N. Murata, Y. Ozawa, K. Irie, T. Shirasawa, and T. Shimizu, Insulin receptor mutation results in insulin resistance and hyperinsulinemia but does not exacerbate Alzheimer's-like phenotypes in mice. Biochem Biophys Res Commun, 2011. 409(1): p. 34-9.

Murakami, K., N. Murata, Y. Ozawa, N. Kinoshita, K. Irie, T. Shirasawa, and T. Shimizu, Vitamin C Restores Behavioral Deficits and Amyloid-beta Oligomerization without Affecting Plaque Formation in a Mouse Model of Alzheimer's Disease. J Alzheimers Dis, 2011. 26(1): p. 7-18.

Murakami, K., N. Murata, Y. Noda, S. Tahara, T. Kaneko, N. Kinoshita, H. Hatsuta, S. Murayama, K.J. Barnham, K. Irie, T. Shirasawa, and T. Shimizu, SOD1 (Copper/Zinc Superoxide Dismutase) Deficiency Drives Amyloid beta Protein Oligomerization and Memory Loss in Mouse Model of Alzheimer Disease. J Biol Chem, 2011. 286(52): p. 44557-68.

Lustgarten, M.S., Y.C. Jang, Y. Liu, W. Qi, Y. Qin, P.L. Dahia, Y. Shi, A. Bhattacharya, F.L. Muller, T. Shimizu, T. Shirasawa, A. Richardson, and H. Van Remmen, MnSOD deficiency results in elevated oxidative stress and decreased mitochondrial function but does not lead to muscle atrophy during aging. Aging Cell, 2011. 10(3): p. 493-505.

Jones, M.K., E. Zhu, E.V. Sarino, O.R. Padilla, T. Takahashi, T. Shimizu, and T. Shirasawa, Loss of parietal cell superoxide dismutase leads to gastric oxidative stress and increased injury susceptibility in mice. Am J Physiol Gastrointest Liver Physiol, 2011. 301(3): p. G537-46.

Case, A.J., J.L. McGill, L.T. Tygrett, T. Shirasawa, D.R. Spitz, T.J. Waldschmidt, K.L. Legge, and F.E. Domann, Elevated mitochondrial superoxide disrupts normal T cell development, impairing adaptive immune responses to an influenza challenge. Free Radic Biol Med, 2011. 50(3): p. 448-58.

Scientific Papers 2010 - 2006

2010

Sunagawa, T., T. Shimizu, T. Kanda, M. Tagashira, M. Sami, and T. Shirasawa, Procyanidins from Apples (Malus pumila Mill.) Extend the Lifespan of Caenorhabditis elegans. Planta Med, 2010.

Shirasawa, T., [Aging theory and aging control]. Nippon Ronen Igakkai Zasshi, 2010. 47(1): p. 24-7.

Shimojo, Y., K. Kosaka, and T. Shirasawa, Effect of Ganoderma lucidum extract on adipocyte differentiation and adiponectin gene expression in the murine pre-adipocyte cell line, 3T3-L1. Phytother Res, 2010.

Shimojo, Y., K. Kosaka, Y. Noda, T. Shimizu, and T. Shirasawa, Effect of rosmarinic acid in motor dysfunction and life span in a mouse model of familial amyotrophic lateral sclerosis. J Neurosci Res, 2010. 88(4): p. 896-904.

Shimizu, T. and T. Shirasawa, [Anti-aging research using Mn-SOD conditional knockout mice]. Yakugaku Zasshi, 2010. 130(1): p. 19-24.

Shimizu, T., H. Nojiri, S. Kawakami, S. Uchiyama, and T. Shirasawa, Model mice for tissue-specific deletion of the manganese superoxide dismutase gene. Geriatr Gerontol Int, 2010. 10 Suppl 1: p. S70-9.

Murata, N., K. Murakami, Y. Ozawa, N. Kinoshita, K. Irie, T. Shirasawa, and T. Shimizu, Silymarin attenuated the amyloid beta plaque burden and improved behavioral abnormalities in an Alzheimer's disease mouse model. Biosci Biotechnol Biochem, 2010. 74(11): p. 2299-306.

Murakami, K., Y. Masuda, T. Shirasawa, T. Shimizu, and K. Irie, The turn formation at positions 22 and 23 in the 42-mer amyloid beta peptide: the emerging role in the pathogenesis of Alzheimer's disease. Geriatr Gerontol Int, 2010. 10 Suppl 1: p. S169-79.

Kuwahara, H., T. Horie, S. Ishikawa, C. Tsuda, S. Kawakami, Y. Noda, T. Kaneko, S. Tahara, T. Tachibana, M. Okabe, J. Melki, R. Takano, T. Toda, D. Morikawa, H. Nojiri, H. Kurosawa, T. Shirasawa, and T. Shimizu, Oxidative stress in skeletal muscle causes severe disturbance of exercise activity without muscle atrophy. Free Radic Biol Med, 2010. 48(9): p. 1252-62.

Kosaka, K., J. Mimura, K. Itoh, T. Satoh, Y. Shimojo, C. Kitajima, A. Maruyama, M. Yamamoto, and T. Shirasawa, Role of Nrf2 and p62/ZIP in the neurite outgrowth by carnosic acid in PC12h cells. J Biochem, 2010. 147(1): p. 73-81.

Kinouchi, T., A. Matsuda, S. Kawakami, T. Shimizu, T. Shirasawa, and N. Fujii, Influence of oxidative stress on D-aspartyl endopeptidase activity. Chem Biodivers, 2010. 7(6): p. 1398-402.

Kim, J., T. Shirasawa, and Y. Miyamoto, The effect of TAT conjugated platinum nanoparticles on lifespan in a nematode Caenorhabditis elegans model. Biomaterials, 2010. 31(22): p. 5849-54.

Case, A.J., J.L. McGill, L.T. Tygrett, T. Shirasawa, D.R. Spitz, T.J. Waldschmidt, K.L. Legge, and F.E. Domann, Elevated mitochondrial superoxide disrupts normal T-cell development to impair adaptive immune responses to an influenza challenge. Free Radic Biol Med, 2010.

2009

Shirasawa, T., [Caloric restriction and anti-aging]. Nippon Ronen Igakkai Zasshi, 2009. 46(3): p. 222-4.

Shimojo, Y., K. Kosaka, Y. Noda, T. Shimizu, and T. Shirasawa, Effect of rosmarinic acid in motor dysfunction and life span in a mouse model of familial amyotrophic lateral sclerosis. J Neurosci Res, 2009.

Shimizu, T. and T. Shirasawa, [Anti-aging effects by caloric restriction]. Nippon Rinsho, 2009. 67(7): p. 1366-71.

Satoh, A., S. Tsuji, Y. Okada, N. Murakami, M. Urami, K. Nakagawa, M. Ishikura, M. Katagiri, Y. Koga, and T. Shirasawa, Preliminary Clinical Evaluation of Toxicity and Efficacy of A New Astaxanthin-rich Haematococcus pluvialis Extract. J Clin Biochem Nutr, 2009. 44(3): p. 280-4.

Murakami, K., J. Inagaki, M. Saito, Y. Ikeda, C. Tsuda, Y. Noda, S. Kawakami, T. Shirasawa, and T. Shimizu, Skin atrophy in cytoplasmic SOD-deficient mice and its complete recovery using a vitamin C derivative. Biochem Biophys Res Commun, 2009. 382(2): p. 457-61.

Masuda, Y., S. Uemura, R. Ohashi, A. Nakanishi, K. Takegoshi, T. Shimizu, T. Shirasawa, and K. Irie, Identification of physiological and toxic conformations in Abeta42 aggregates. Chembiochem, 2009. 10(2): p. 287-95.

Lustgarten, M.S., Y.C. Jang, Y. Liu, F.L. Muller, W. Qi, M. Steinhelper, S.V. Brooks, L. Larkin, T. Shimizu, T. Shirasawa, L.M. McManus, A. Bhattacharya, A. Richardson, and H. Van Remmen, Conditional knockout of Mn-SOD targeted to type IIB skeletal muscle fibers increases oxidative stress and is sufficient to alter aerobic exercise capacity. Am J Physiol Cell Physiol, 2009. 297(6): p. C1520-32.

Kosaka, K., J. Mimura, K. Itoh, T. Satoh, Y. Shimojo, C. Kitajima, A. Maruyama, M. Yamamoto, and T. Shirasawa, Role of Nrf2 and p62/ZIP in the neurite outgrowth by carnosic acid in PC12h cells. J Biochem, 2009. 147(1): p. 73-81.

Kawakami, S., A. Matsuda, T. Sunagawa, Y. Noda, T. Kaneko, S. Tahara, Y. Hiraumi, S. Adachi, H. Matsui, K. Ando, T. Fujita, N. Maruyama, T. Shirasawa, and T. Shimizu, Antioxidant, EUK-8, prevents murine dilated cardiomyopathy. Circ J, 2009. 73(11): p. 2125-34.

Chiba, T., Y. Kamei, T. Shimizu, T. Shirasawa, A. Katsumata, L. Shiraishi, S. Sugita, Y. Ogawa, S. Miura, and O. Ezaki, Overexpression of FOXO1 in skeletal muscle does not alter longevity in mice. Mech Ageing Dev, 2009. 130(7): p. 420-8.

2008

Watanabe, T., T. Takeda, S. Omiya, S. Hikoso, O. Yamaguchi, Y. Nakano, Y. Higuchi, A. Nakai, Y. Abe, Y. Aki-Jin, M. Taniike, I. Mizote, Y. Matsumura, T. Shimizu, K. Nishida, K. Imai, M. Hori, T. Shirasawa, and K. Otsu, Reduction in hemoglobin-oxygen affinity results in the improvement of exercise capacity in mice with chronic heart failure. J Am Coll Cardiol, 2008. 52(9): p. 779-86.

Takahashi, M., T. Shimizu, E. Moriizumi, and T. Shirasawa, Clk-1 deficiency induces apoptosis associated with mitochondrial dysfunction in mouse embryos. Mech Ageing Dev, 2008. 129(5): p. 291-8.

Shimizu, T. and T. Shirasawa, [The present and future of antiaging]. Gan To Kagaku Ryoho, 2008. 35(1): p. 1-5.

Satoh, T., K. Kosaka, K. Itoh, A. Kobayashi, M. Yamamoto, Y. Shimojo, C. Kitajima, J. Cui, J. Kamins, S. Okamoto, M. Izumi, T. Shirasawa, and S.A. Lipton, Carnosic acid, a catechol-type electrophilic compound, protects neurons both in vitro and in vivo through activation of the Keap1/Nrf2 pathway via S-alkylation of targeted cysteines on Keap1. J Neurochem, 2008. 104(4): p. 1116-31.

Satoh, T., M. Izumi, Y. Inukai, Y. Tsutsumi, N. Nakayama, K. Kosaka, Y. Shimojo, C. Kitajima, K. Itoh, T. Yokoi, and T. Shirasawa, Carnosic acid protects neuronal HT22 Cells through activation of the antioxidant-responsive element in free carboxylic acid- and catechol hydroxyl moieties-dependent manners. Neurosci Lett, 2008. 434(3): p. 260-5.

Murakami, K., M. Uno, Y. Masuda, T. Shimizu, T. Shirasawa, and K. Irie, Isomerization and/or racemization at Asp23 of Abeta42 do not increase its aggregative ability, neurotoxicity, and radical productivity in vitro. Biochem Biophys Res Commun, 2008. 366(3): p. 745-51.

Masuda, Y., S. Uemura, A. Nakanishi, R. Ohashi, K. Takegoshi, T. Shimizu, T. Shirasawa, and K. Irie, Verification of the C-terminal intramolecular beta-sheet in Abeta42 aggregates using solid-state NMR: implications for potent neurotoxicity through the formation of radicals. Bioorg Med Chem Lett, 2008. 18(11): p. 3206-10.

Masuda, Y., A. Nakanishi, R. Ohashi, K. Takegoshi, T. Shimizu, T. Shirasawa, and K. Irie, Verification of the intermolecular parallel beta-sheet in E22K-Abeta42 aggregates by solid-state NMR using rotational resonance: implications for the supramolecular arrangement of the toxic conformer of Abeta42. Biosci Biotechnol Biochem, 2008. 72(8): p. 2170-5.

Kosugi, S., T. Furuchi, M. Katane, M. Sekine, T. Shirasawa, and H. Homma, Suppression of protein l-isoaspartyl (d-aspartyl) methyltransferase results in hyperactivation of EGF-stimulated MEK-ERK signaling in cultured mammalian cells. Biochem Biophys Res Commun, 2008. 371(1): p. 22-7.

Kim, J., M. Takahashi, T. Shimizu, T. Shirasawa, M. Kajita, A. Kanayama, and Y. Miyamoto, Effects of a potent antioxidant, platinum nanoparticle, on the lifespan of Caenorhabditis elegans. Mech Ageing Dev, 2008. 129(6): p. 322-31.

Ikeda, Y., S. Tsuji, A. Satoh, M. Ishikura, T. Shirasawa, and T. Shimizu, Protective effects of astaxanthin on 6-hydroxydopamine-induced apoptosis in human neuroblastoma SH-SY5Y cells. J Neurochem, 2008. 107(6): p. 1730-40.

Hashizume, K., M. Hirasawa, Y. Imamura, S. Noda, T. Shimizu, K. Shinoda, T. Kurihara, K. Noda, Y. Ozawa, S. Ishida, Y. Miyake, T. Shirasawa, and K. Tsubota, Retinal dysfunction and progressive retinal cell death in SOD1-deficient mice. Am J Pathol, 2008. 172(5): p. 1325-31.

2007

Oda, K., N. Tanaka, T. Arai, J. Araki, Y. Song, L. Zhang, A. Kuchiba, T. Hosoi, T. Shirasawa, M. Muramatsu, and M. Sawabe, Polymorphisms in pro- and anti-inflammatory cytokine genes and susceptibility to atherosclerosis: a pathological study of 1503 consecutive autopsy cases. Hum Mol Genet, 2007. 16(6): p. 592-9.

Irie, K., K. Murakami, Y. Masuda, A. Morimoto, H. Ohigashi, H. Hara, R. Ohashi, K. Takegoshi, H. Fukuda, M. Nagao, T. Shimizu, and T. Shirasawa, The toxic conformation of the 42-residue amyloid beta peptide and its relevance to oxidative stress in Alzheimer's disease. Mini Rev Med Chem, 2007. 7(10): p. 1001-8.

2006

Uchiyama, S., T. Shimizu, and T. Shirasawa, CuZn-SOD deficiency causes ApoB degradation and induces hepatic lipid accumulation by impaired lipoprotein secretion in mice. J Biol Chem, 2006. 281(42): p. 31713-9.

Shirasawa, T. and T. Shimizu, Insulin-like signal regulating the lifespan ot the animals. Seikagaku, 2006. 78(3): p. 181-188.

Shirasawa, T. and T. Shimizu, [Insulin-like signal regulating the life span of the animals]. Seikagaku, 2006. 78(3): p. 181-8.

Sawabe, M., M. Saito, M. Naka, I. Kasahara, Y. Saito, T. Arai, A. Hamamatsu, and T. Shirasawa, Standard organ weights among elderly Japanese who died in hospital, including 50 centenarians. Pathol Int, 2006. 56(6): p. 315-23.

Ogino, J., K. Sakurai, K. Yoshiwara, Y. Suzuki, N. Ishizuka, N. Seki, H. Koseki, T. Shirasawa, N. Hashimoto, K. Yagui, and Y. Saito, Insulin resistance and increased pancreatic beta-cell proliferation in mice expressing a mutant insulin receptor (P1195L). J Endocrinol, 2006. 190(3): p. 739-47.

Nojiri, H., T. Shimizu, M. Funakoshi, O. Yamaguchi, H. Zhou, S. Kawakami, Y. Ohta, M. Sami, T. Tachibana, H. Ishikawa, H. Kurosawa, R.C. Kahn, K. Otsu, and T. Shirasawa, Oxidative stress causes heart failure with impaired mitochondrial respiration. J Biol Chem, 2006. 281(44): p. 33789-801.

Nakajima, M., M. Ogawa, Y. Shimoda, H. Koseki, T. Shirasawa, and K. Furukawa, Accelerated acquisition of permeability barrier function in the skin of presenilin-1-deficient embryos. Arch Dermatol Res, 2006. 298(7): p. 339-45.

Nakajima, M., M. Ogawa, Y. Shimoda, S. Hiraoka, M. Iida, H. Koseki, T. Shirasawa, and K. Furukawa, Presenilin-1 controls the growth and differentiation of endothelial progenitor cells through its beta-catenin-binding region. Cell Biol Int, 2006. 30(3): p. 239-43.

Murakami, K., K. Namikawa, T. Shimizu, T. Shirasawa, S. Yoshida, and H. Kiyama, Nerve injury induces the expression of EXT2, a glycosyltransferase required for heparan sulfate synthesis. Neuroscience, 2006. 141(4): p. 1961-9.

Misawa, H., K. Nakata, J. Matsuura, Y. Moriwaki, K. Kawashima, T. Shimizu, T. Shirasawa, and R. Takahashi, Conditional knockout of Mn superoxide dismutase in postnatal motor neurons reveals resistance to mitochondrial generated superoxide radicals. Neurobiol Dis, 2006. 23(1): p. 169-77.

Imamura, Y., S. Noda, K. Hashizume, K. Shinoda, M. Yamaguchi, S. Uchiyama, T. Shimizu, Y. Mizushima, T. Shirasawa, and K. Tsubota, Drusen, choroidal neovascularization, and retinal pigment epithelium dysfunction in SOD1-deficient mice: a model of age-related macular degeneration. Proc Natl Acad Sci U S A, 2006. 103(30): p. 11282-7.

Scientific Papers 2005 - 2001

2005

Uchiyama, S., H. Koike, T. Shimizu, and T. Shirasawa, A superoxide disumutase/catalase mimetic extends the lifespan of short-lived mev-1 mutant but not the wild type strain in Caenorhabditis elegans. Anti-Aging Med Res, 2005. 2(1): p. 39-47.

Shimizu, T., Y. Matsuoka, and T. Shirasawa, Biological significance of isoaspartate and its repair system. Biol Pharm Bull, 2005. 28(9): p. 1590-6.

Murakami, K., K. Irie, H. Ohigashi, H. Hara, M. Nagao, T. Shimizu, and T. Shirasawa, Formation and stabilization model of the 42-mer Abeta radical: implications for the long-lasting oxidative stress in Alzheimer's disease. J Am Chem Soc, 2005. 127(43): p. 15168-74.

Masuda, Y., K. Irie, K. Murakami, H. Ohigashi, R. Ohashi, K. Takegoshi, T. Shimizu, and T. Shirasawa, Verification of the turn at positions 22 and 23 of the beta-amyloid fibrils with Italian mutation using solid-state NMR. Bioorg Med Chem, 2005. 13(24): p. 6803-9.

Irie, K., K. Murakami, Y. Masuda, A. Morimoto, H. Ohigashi, R. Ohashi, K. Takegoshi, M. Nagao, T. Shimizu, and T. Shirasawa, Structure of beta-amyloid fibrils and its relevance to their neurotoxicity: implications for the pathogenesis of Alzheimer's disease. J Biosci Bioeng, 2005. 99(5): p. 437-47.

Huang, F., T. Shimizu, A. Ishihara, S. Yuasa, K. Nishimura, T. Kugimiya, and T. Shirasawa, Tissue hyperoxygenation promotes oxidative metabolism in motor unit. J Neurosci Res, 2005. 80(4): p. 584-91.

Huang, F., H. Nojiri, T. Shimizu, and T. Shirasawa, Beneficial effect of transfusion with low-affinity red blood cells in endotoxemia. Transfusion, 2005. 45(11): p. 1785-90.

Baba, T., T. Shimizu, Y. Suzuki, M. Ogawara, K. Isono, H. Koseki, H. Kurosawa, and T. Shirasawa, Estrogen, insulin, and dietary signals cooperatively regulate longevity signals to enhance resistance to oxidative stress in mice. J Biol Chem, 2005. 280(16): p. 16417-26.

2004

Nakajima, M. and T. Shirasawa, Presenilin-1-deficient neurons are nitric oxide-dependently killed by hydrogen peroxide in vitro. Neuroscience, 2004. 125(3): p. 563-8.

Nakajima, M., E. Moriizumi, H. Koseki, and T. Shirasawa, Presenilin 1 is essential for cardiac morphogenesis. Dev Dyn, 2004. 230(4): p. 795-9.

Nakai, D., T. Shimizu, H. Nojiri, S. Uchiyama, H. Koike, M. Takahashi, K. Hirokawa, and T. Shirasawa, coq7/clk-1 regulates mitochondrial respiration and the generation of reactive oxygen species via coenzyme Q. Aging Cell, 2004. 3(5): p. 273-81.

Morimoto, A., K. Irie, K. Murakami, Y. Masuda, H. Ohigashi, M. Nagao, H. Fukuda, T. Shimizu, and T. Shirasawa, Analysis of the secondary structure of beta-amyloid (Abeta42) fibrils by systematic proline replacement. J Biol Chem, 2004. 279(50): p. 52781-8.

2003

Takahashi, T., K. Takahashi, P.L. St John, P.A. Fleming, T. Tomemori, T. Watanabe, D.R. Abrahamson, C.J. Drake, T. Shirasawa, and T.O. Daniel, A mutant receptor tyrosine phosphatase, CD148, causes defects in vascular development. Mol Cell Biol, 2003. 23(5): p. 1817-31.

Shirasawa, T., M. Izumizaki, Y. Suzuki, A. Ishihara, T. Shimizu, M. Tamaki, F. Huang, K. Koizumi, M. Iwase, H. Sakai, E. Tsuchida, K. Ueshima, H. Inoue, H. Koseki, T. Senda, T. Kuriyama, and I. Homma, Oxygen affinity of hemoglobin regulates O2 consumption, metabolism, and physical activity. J Biol Chem, 2003. 278(7): p. 5035-43.

Shirasawa, T., [Longevity and genes]. Nippon Ronen Igakkai Zasshi, 2003. 40(6): p. 596-8.

Shin, Y., H.S. Cho, H. Fukumoto, T. Shimizu, T. Shirasawa, S.M. Greenberg, and G.W. Rebeck, Abeta species, including IsoAsp23 Abeta, in Iowa-type familial cerebral amyloid angiopathy. Acta Neuropathol, 2003. 105(3): p. 252-8.

Shin, R.M., M. Masuda, M. Miura, H. Sano, T. Shirasawa, W.J. Song, K. Kobayashi, and T. Aosaki, Dopamine D4 receptor-induced postsynaptic inhibition of GABAergic currents in mouse globus pallidus neurons. J Neurosci, 2003. 23(37): p. 11662-72.

Nakajima, M., S. Yuasa, M. Ueno, N. Takakura, H. Koseki, and T. Shirasawa, Abnormal blood vessel development in mice lacking presenilin-1. Mech Dev, 2003. 120(6): p. 657-67.

Murakami, K., K. Irie, A. Morimoto, H. Ohigashi, M. Shindo, M. Nagao, T. Shimizu, and T. Shirasawa, Neurotoxicity and physicochemical properties of Abeta mutant peptides from cerebral amyloid angiopathy: implication for the pathogenesis of cerebral amyloid angiopathy and Alzheimer's disease. J Biol Chem, 2003. 278(46): p. 46179-87.

Morio, H., Y. Honda, H. Toyoda, M. Nakajima, H. Kurosawa, and T. Shirasawa, EXT gene family member rib-2 is essential for embryonic development and heparan sulfate biosynthesis in Caenorhabditis elegans. Biochem Biophys Res Commun, 2003. 301(2): p. 317-23.

Izumizaki, M., M. Tamaki, Y. Suzuki, M. Iwase, T. Shirasawa, H. Kimura, and I. Homma, The affinity of hemoglobin for oxygen affects ventilatory responses in mutant mice with Presbyterian hemoglobinopathy. Am J Physiol Regul Integr Comp Physiol, 2003. 285(4): p. R747-53.

2002

Yuasa, S., M. Nakajima, H. Aizawa, N. Sahara, K. Koizumi, T. Sakai, M. Usami, S. Kobayashi, H. Kuroyanagi, H. Mori, H. Koseki, and T. Shirasawa, Impaired cell cycle control of neuronal precursor cells in the neocortical primordium of presenilin-1-deficient mice. J Neurosci Res, 2002. 70(3): p. 501-13.

Suzuki, Y., T. Shimizu, H. Sakai, M. Tamaki, K. Koizumi, T. Kuriyama, E. Tsuchida, H. Koseki, and T. Shirasawa, Model mice for Presbyterian hemoglobinopathy (Asn(beta108)-->Lys) confer hemolytic anemia with altered oxygen affinity and instability of Hb. Biochem Biophys Res Commun, 2002. 295(4): p. 869-76.

Smith, C.D., M. Carson, A.M. Friedman, M.M. Skinner, L. Delucas, L. Chantalat, L. Weise, T. Shirasawa, and D. Chattopadhyay, Crystal structure of human L-isoaspartyl-O-methyl-transferase with S-adenosyl homocysteine at 1.6-A resolution and modeling of an isoaspartyl-containing peptide at the active site. Protein Sci, 2002. 11(3): p. 625-35.

Shimizu, T., T. Ikegami, M. Ogawara, Y. Suzuki, M. Takahashi, H. Morio, and T. Shirasawa, Transgenic expression of the protein-L-isoaspartyl methyltransferase (PIMT) gene in the brain rescues mice from the fatal epilepsy of PIMT deficiency. J Neurosci Res, 2002. 69(3): p. 341-52.

Shimizu, T., H. Fukuda, S. Murayama, N. Izumiyama, and T. Shirasawa, Isoaspartate formation at position 23 of amyloid beta peptide enhanced fibril formation and deposited onto senile plaques and vascular amyloids in Alzheimer's disease. J Neurosci Res, 2002. 70(3): p. 451-61.

Ogawara, M., M. Takahashi, T. Shimizu, M. Nakajima, Y. Setoguchi, and T. Shirasawa, Adenoviral expression of protein-L-isoaspartyl methyltransferase (PIMT) partially attenuates the biochemical changes in PIMT-deficient mice. J Neurosci Res, 2002. 69(3): p. 353-61.

Odaka, C., T. Mizuochi, T. Shirasawa, P. Morain, and F. Checler, Murine T cells expressing high activity of prolyl endopeptidase are susceptible to activation-induced cell death. FEBS Lett, 2002. 512(1-3): p. 163-7.

Murakami, K., K. Irie, A. Morimoto, H. Ohigashi, M. Shindo, M. Nagao, T. Shimizu, and T. Shirasawa, Synthesis, aggregation, neurotoxicity, and secondary structure of various A beta 1-42 mutants of familial Alzheimer's disease at positions 21-23. Biochem Biophys Res Commun, 2002. 294(1): p. 5-10.

Morimoto, K., T. Shimizu, K. Furukawa, H. Morio, H. Kurosawa, and T. Shirasawa, Transgenic expression of the EXT2 gene in developing chondrocytes enhances the synthesis of heparan sulfate and bone formation in mice. Biochem Biophys Res Commun, 2002. 292(4): p. 999-1009.

Morimoto, A., K. Irie, K. Murakami, H. Ohigashi, M. Shindo, M. Nagao, T. Shimizu, and T. Shirasawa, Aggregation and neurotoxicity of mutant amyloid beta (A beta) peptides with proline replacement: importance of turn formation at positions 22 and 23. Biochem Biophys Res Commun, 2002. 295(2): p. 306-11.

Ishigami, A., T. Fujita, S. Handa, T. Shirasawa, H. Koseki, T. Kitamura, N. Enomoto, N. Sato, T. Shimosawa, and N. Maruyama, Senescence marker protein-30 knockout mouse liver is highly susceptible to tumor necrosis factor-alpha- and Fas-mediated apoptosis. Am J Pathol, 2002. 161(4): p. 1273-81.

Ikegami, T., Y. Suzuki, T. Shimizu, K. Isono, H. Koseki, and T. Shirasawa, Model mice for tissue-specific deletion of the manganese superoxide dismutase (MnSOD) gene. Biochem Biophys Res Commun, 2002. 296(3): p. 729-36.

2001

Tomita, K., K. Saijo, S. Yamasaki, T. Iida, F. Nakatsu, H. Arase, H. Ohno, T. Shirasawa, T. Kuriyama, J.J. O'Shea, and T. Saito, Cytokine-independent Jak3 activation upon T cell receptor (TCR) stimulation through direct association of Jak3 and the TCR complex. J Biol Chem, 2001. 276(27): p. 25378-85.

Takahashi, M., S. Asaumi, S. Honda, Y. Suzuki, D. Nakai, H. Kuroyanagi, T. Shimizu, Y. Honda, and T. Shirasawa, Mouse coq7/clk-1 orthologue rescued slowed rhythmic behavior and extended life span of clk-1 longevity mutant in Caenorhabditis elegans. Biochem Biophys Res Commun, 2001. 286(3): p. 534-40.

Nakajima, M., M. Miura, T. Aosaki, and T. Shirasawa, Deficiency of presenilin-1 increases calcium-dependent vulnerability of neurons to oxidative stress in vitro. J Neurochem, 2001. 78(4): p. 807-14.

Nakai, D., S. Yuasa, M. Takahashi, T. Shimizu, S. Asaumi, K. Isono, T. Takao, Y. Suzuki, H. Kuroyanagi, K. Hirokawa, H. Koseki, and T. Shirasawa, Mouse homologue of coq7/clk-1, longevity gene in Caenorhabditis elegans, is essential for coenzyme Q synthesis, maintenance of mitochondrial integrity, and neurogenesis. Biochem Biophys Res Commun, 2001. 289(2): p. 463-71.

Koizumi, K., M. Nakajima, S. Yuasa, Y. Saga, T. Sakai, T. Kuriyama, T. Shirasawa, and H. Koseki, The role of presenilin 1 during somite segmentation. Development, 2001. 128(8): p. 1391-402.

Ikegaya, Y., M. Yamada, T. Fukuda, H. Kuroyanagi, T. Shirasawa, and N. Nishiyama, Aberrant synaptic transmission in the hippocampal CA3 region and cognitive deterioration in protein-repair enzyme-deficient mice. Hippocampus, 2001. 11(3): p. 287-98.

Scientific Papers 2000 - 1996

2000

Tomemori, T., N. Seki, Y. Suzuki, T. Shimizu, H. Nagata, A. Konno, and T. Shirasawa, Isolation and characterization of murine orthologue of PTP-BK. Biochem Biophys Res Commun, 2000. 276(3): p. 974-81.

Shimizu, T., A. Watanabe, M. Ogawara, H. Mori, and T. Shirasawa, Isoaspartate formation and neurodegeneration in Alzheimer's disease. Arch Biochem Biophys, 2000. 381(2): p. 225-34.

Nakajima, M., T. Shimizu, and T. Shirasawa, Notch-1 activation by familial Alzheimer's disease (FAD)-linked mutant forms of presenilin-1. J Neurosci Res, 2000. 62(2): p. 311-7.

Kobayashi, S., K. Morimoto, T. Shimizu, M. Takahashi, H. Kurosawa, and T. Shirasawa, Association of EXT1 and EXT2, hereditary multiple exostoses gene products, in Golgi apparatus. Biochem Biophys Res Commun, 2000. 268(3): p. 860-7.

Gonzalez, L.J., T. Shimizu, Y. Satomi, L. Betancourt, V. Besada, G. Padron, R. Orlando, T. Shirasawa, Y. Shimonishi, and T. Takao, Differentiating alpha- and beta-aspartic acids by electrospray ionization and low-energy tandem mass spectrometry. Rapid Commun Mass Spectrom, 2000. 14(22): p. 2092-102.

1999

Yan, J., H. Kuroyanagi, T. Tomemori, N. Okazaki, K. Asato, Y. Matsuda, Y. Suzuki, Y. Ohshima, S. Mitani, Y. Masuho, T. Shirasawa, and M. Muramatsu, Mouse ULK2, a novel member of the UNC-51-like protein kinases: unique features of functional domains. Oncogene, 1999. 18(43): p. 5850-9.

Tomoda, T., R.S. Bhatt, H. Kuroyanagi, T. Shirasawa, and M.E. Hatten, A mouse serine/threonine kinase homologous to C. elegans UNC51 functions in parallel fiber formation of cerebellar granule neurons. Neuron, 1999. 24(4): p. 833-46.

Shirasawa, T., [Characterization of protein isoaspartyl methyltransferase (PIMT)-deficient mice]. Seikagaku, 1999. 71(2): p. 134-40.

Ohno, H., T. Tomemori, F. Nakatsu, Y. Okazaki, R.C. Aguilar, H. Foelsch, I. Mellman, T. Saito, T. Shirasawa, and J.S. Bonifacino, Mu1B, a novel adaptor medium chain expressed in polarized epithelial cells. FEBS Lett, 1999. 449(2-3): p. 215-20.

Nakano, H., S. Sakon, H. Koseki, T. Takemori, K. Tada, M. Matsumoto, E. Munechika, T. Sakai, T. Shirasawa, H. Akiba, T. Kobata, S.M. Santee, C.F. Ware, P.D. Rennert, M. Taniguchi, H. Yagita, and K. Okumura, Targeted disruption of Traf5 gene causes defects in CD40- and CD27-mediated lymphocyte activation. Proc Natl Acad Sci U S A, 1999. 96(17): p. 9803-8.

Fukuda, H., T. Shimizu, M. Nakajima, H. Mori, and T. Shirasawa, Synthesis, aggregation, and neurotoxicity of the Alzheimer's Abeta1-42 amyloid peptide and its isoaspartyl isomers. Bioorg Med Chem Lett, 1999. 9(7): p. 953-6.

Fujita, T., T. Shirasawa, and N. Maruyama, Expression and structure of senescence marker protein-30 (SMP30) and its biological significance. Mech Ageing Dev, 1999. 107(3): p. 271-80.

Atsuta, R., K. Akiyama, T. Shirasawa, K. Okumura, Y. Fukuchi, and C. Ra, Atopic asthma is dominant in elderly onset asthmatics: possibility for an alteration of mast cell function by aging through Fc receptor expression. Int Arch Allergy Immunol, 1999. 120 Suppl 1: p. 76-81.

Asaumi, S., H. Kuroyanagi, N. Seki, and T. Shirasawa, Orthologues of the Caenorhabditis elegans longevity gene clk-1 in mouse and human. Genomics, 1999. 58(3): p. 293-301.

Akashi, T., T. Miyagi, N. Ando, Y. Suzuki, T. Nemoto, Y. Eishi, K. Nakamura, T. Shirasawa, N. Osa, N. Tanaka, and R.E. Burgeson, Synthesis of basement membrane by gastrointestinal cancer cell lines. J Pathol, 1999. 187(2): p. 223-8.

1998

Yan, J., H. Kuroyanagi, A. Kuroiwa, Y. Matsuda, H. Tokumitsu, T. Tomoda, T. Shirasawa, and M. Muramatsu, Identification of mouse ULK1, a novel protein kinase structurally related to C. elegans UNC-51. Biochem Biophys Res Commun, 1998. 246(1): p. 222-7.

Yamamoto, A., H. Takagi, D. Kitamura, H. Tatsuoka, H. Nakano, H. Kawano, H. Kuroyanagi, Y. Yahagi, S. Kobayashi, K. Koizumi, T. Sakai, K. Saito, T. Chiba, K. Kawamura, K. Suzuki, T. Watanabe, H. Mori, and T. Shirasawa, Deficiency in protein L-isoaspartyl methyltransferase results in a fatal progressive epilepsy. J Neurosci, 1998. 18(6): p. 2063-74.

Suzuki, G., Y. Nakata, Y. Dan, A. Uzawa, K. Nakagawa, T. Saito, K. Mita, and T. Shirasawa, Loss of SDF-1 receptor expression during positive selection in the thymus. Int Immunol, 1998. 10(8): p. 1049-56.

Shindo, M., H. Nakano, H. Kuroyanagi, T. Shirasawa, M. Mihara, D.J. Gilbert, N.A. Jenkins, N.G. Copeland, H. Yagita, and K. Okumura, cDNA cloning, expression, subcellular localization, and chromosomal assignment of mammalian aurora homologues, aurora-related kinase (ARK) 1 and 2. Biochem Biophys Res Commun, 1998. 244(1): p. 285-92.

Kuroyanagi, H., J. Yan, N. Seki, Y. Yamanouchi, Y. Suzuki, T. Takano, M. Muramatsu, and T. Shirasawa, Human ULK1, a novel serine/threonine kinase related to UNC-51 kinase of Caenorhabditis elegans: cDNA cloning, expression, and chromosomal assignment. Genomics, 1998. 51(1): p. 76-85.

Kuroyanagi, H., M. Tagawa, and T. Shirasawa, [Structure, expression and functions of receptor-type protein tyrosine phosphatase RPTP-BK in central nervous system]. Tanpakushitsu Kakusan Koso, 1998. 43(8 Suppl): p. 1162-8.

Fujita, T., T. Shirasawa, H. Inoue, T. Kitamura, and N. Maruyama, Hepatic and renal expression of senescence marker protein-30 and its biological significance. J Gastroenterol Hepatol, 1998. 13 Suppl: p. S124-31.

1997

Tagawa, M., T. Shirasawa, Y. Yahagi, T. Tomoda, H. Kuroyanagi, S. Fujimura, S. Sakiyama, and N. Maruyama, Identification of a receptor-type protein tyrosine phosphatase expressed in postmitotic maturing neurons: its structure and expression in the central nervous system. Biochem J, 1997. 321 ( Pt 3): p. 865-71.

Smith, C.D., J. Barchue, C. Mentel, L. DeLucas, T. Shirasawa, and D. Chattopadhyay, Crystallization and preliminary cryogenic X-ray diffraction analyses of protein L-isoaspartyl O-methyltransferase from human fetal brain. Proteins, 1997. 28(3): p. 457-60.

Shimizu, E., H. Shirasawa, K. Kodama, H. Kuroyanagi, T. Shirasawa, T. Sato, and B. Simizu, Glutamate dehydrogenase mRNA is immediately induced after phencyclidine treatment in the rat brain. Schizophr Res, 1997. 25(3): p. 251-8.

Sakai, T., H. Tanaka, and T. Shirasawa, Two distinct epithelial responses may compensate for the ureteral obstruction in early and late phase of unilateral ureteral obstruction-treated rat: cellular proliferation in acute phase and osteopontin expression in chronic phase. Nephron, 1997. 77(3): p. 340-5.

Sakai, T., T. Kawamura, and T. Shirasawa, Mizoribine improves renal tubulointerstitial fibrosis in unilateral ureteral obstruction (UUO)-treated rat by inhibiting the infiltration of macrophages and the expression of alpha-smooth muscle actin. J Urol, 1997. 158(6): p. 2316-22.

Mukouyama, Y., H. Kuroyanagi, T. Shirasawa, T. Tomoda, D. Saffen, M. Oishi, and T. Watanabe, Induction of protein tyrosine phosphatase epsilon transcripts during NGF-induced neuronal differentiation of PC12D cells and during the development of the cerebellum. Brain Res Mol Brain Res, 1997. 50(1-2): p. 230-6.

Karasawa, T., T. Shirasawa, Y. Okawa, A. Kuramoto, N. Shimada, Y. Aizawa, M. Zeniya, and G. Toda, Association between frequency of amino acid changes in core region of hepatitis B virus (HBV) and the presence of precore mutation in Japanese HBV carriers. J Gastroenterol, 1997. 32(5): p. 611-22.

Hishikawa, T., H. Ogasawara, H. Kaneko, T. Shirasawa, Y. Matsuura, I. Sekigawa, Y. Takasaki, H. Hashimoto, S. Hirose, S. Handa, R. Nagasawa, and N. Maruyama, Detection of antibodies to a recombinant gag protein derived from human endogenous retrovirus clone 4-1 in autoimmune diseases. Viral Immunol, 1997. 10(3): p. 137-47.

1996

Yamamoto, A., N. Sahara, M. Usami, M. Okochi, T. Kondo, F. Kametani, K. Tanaka, Y. Yahagi, T. Shirasawa, Y. Itoyama, and H. Mori, Abnormal gel-electrophoretic behavior of presenilin 1 and it's fragment. Biochem Biophys Res Commun, 1996. 226(2): p. 536-41.

Yahagi, Y., M. Tagawa, T. Tomoda, and T. Shirasawa, Binary expression of olfactory bulb-protein tyrosine phosphatase in rat central nervous system: developmental gene regulation in neonate cerebral cortex and constitutive expression in olfactory-rhinencephalon. Neurosci Lett, 1996. 211(2): p. 125-8.

Tomoda, T., T. Shirasawa, Y.I. Yahagi, K. Ishii, H. Takagi, Y. Furiya, K.I. Arai, H. Mori, and M.A. Muramatsu, Transforming growth factor-beta is a survival factor for neonate cortical neurons: coincident expression of type I receptors in developing cerebral cortices. Dev Biol, 1996. 179(1): p. 79-90.

Takano, T., Y. Yamanouchi, N. Sahara, T. Shirasawa, and H. Mori, Assignment of Alzheimer's presenilin-1 (PS-1) gene to 14q24.3 by fluorescence in situ hybridization. Neurosci Lett, 1996. 214(1): p. 69-71.

Takahashi, T., T. Shirasawa, K. Miyake, Y. Yahagi, O. Matsumura, A. Abe, Y. Natori, H. Yamabe, Y. Utsunomiya, N. Maruyama, T. Mitarai, and O. Sakai, Jak3 expression in glomerular epithelia of IgA nephropathy (IgA-N) patients. Clin Exp Immunol, 1996. 104(3): p. 517-24.

Sahara, N., Y. Yahagi, H. Takagi, T. Kondo, M. Okochi, M. Usami, T. Shirasawa, and H. Mori, Identification and characterization of presenilin I-467, I-463 and I-374. FEBS Lett, 1996. 381(1-2): p. 7-11.

Kondo, T., T. Shirasawa, Y. Itoyama, and H. Mori, Embryonic genes expressed in Alzheimer's disease brains. Neurosci Lett, 1996. 209(3): p. 157-60.

Fujita, T., T. Shirasawa, K. Uchida, and N. Maruyama, Gene regulation of senescence marker protein-30 (SMP30): coordinated up-regulation with tissue maturation and gradual down-regulation with aging. Mech Ageing Dev, 1996. 87(3): p. 219-29.

Fujita, T., T. Shirasawa, and N. Maruyama, Isolation and characterization of genomic and cDNA clones encoding mouse senescence marker protein-30 (SMP30). Biochim Biophys Acta, 1996. 1308(1): p. 49-57.

Scientific Papers 1995 - 1991

1995

Watanabe, K., T. Fukuchi, H. Hosoya, T. Shirasawa, K. Matuoka, H. Miki, and T. Takenawa, Splicing isoforms of rat Ash/Grb2. Isolation and characterization of the cDNA and genomic DNA clones and implications for the physiological roles of the isoforms. J Biol Chem, 1995. 270(23): p. 13733-9.

Takahashi, T., T. Shirasawa, K. Miyake, Y. Yahagi, N. Maruyama, N. Kasahara, T. Kawamura, O. Matsumura, T. Mitarai, and O. Sakai, Protein tyrosine kinases expressed in glomeruli and cultured glomerular cells: Flt-1 and VEGF expression in renal mesangial cells. Biochem Biophys Res Commun, 1995. 209(1): p. 218-26.

Shirasawa, T., R. Endoh, Y.X. Zeng, K. Sakamoto, and H. Mori, Protein L-isoaspartyl methyltransferase: developmentally regulated gene expression and protein localization in the central nervous system of aged rat. Neurosci Lett, 1995. 188(1): p. 37-40.

Park, S.Y., K. Saijo, T. Takahashi, M. Osawa, H. Arase, N. Hirayama, K. Miyake, H. Nakauchi, T. Shirasawa, and T. Saito, Developmental defects of lymphoid cells in Jak3 kinase-deficient mice. Immunity, 1995. 3(6): p. 771-82.

Karasawa, T., Y. Aizawa, M. Zeniya, A. Kuramoto, T. Shirasawa, and G. Toda, Genetic heterogeneity in the precore region of hepatitis B virus in hepatitis B e antigen-negative chronic hepatitis B patients: spontaneous seroconversion and interferon-induced seroconversion. J Med Virol, 1995. 45(4): p. 373-80.

Ishijima, S.A., Y.X. Zeng, C. Kurashima, M. Utsuyama, T. Shirasawa, K. Sakamoto, and K. Hirokawa, Expression of ZAP-70 gene in the developing thymus and various nonlymphoid tissues of embryonic and adult mice. Cell Immunol, 1995. 165(2): p. 278-83.

Hosoya, H., K. Shimazaki, S. Kobayashi, H. Takahashi, T. Shirasawa, T. Takenawa, and K. Watanabe, Developmental expression of the neural adhesion molecule F3 in the rat brain. Neurosci Lett, 1995. 186(2-3): p. 83-6.

Fujita, T., J.L. Mandel, T. Shirasawa, O. Hino, T. Shirai, and N. Maruyama, Isolation of cDNA clone encoding human homologue of senescence marker protein-30 (SMP30) and its location on the X chromosome. Biochim Biophys Acta, 1995. 1263(3): p. 249-52.

1994

Tomiyama, T., S. Asano, Y. Furiya, T. Shirasawa, N. Endo, and H. Mori, Racemization of Asp23 residue affects the aggregation properties of Alzheimer amyloid beta protein analogues. J Biol Chem, 1994. 269(14): p. 10205-8.

Takahashi, T. and T. Shirasawa, Molecular cloning of rat JAK3, a novel member of the JAK family of protein tyrosine kinases. FEBS Lett, 1994. 342(2): p. 124-8.

Tagawa, M., T. Shirasawa, S. Fujimura, and S. Sakiyama, Expression of protein tyrosine phosphatase genes in the developing brain of mouse and rat. Biochem Mol Biol Int, 1994. 33(6): p. 1221-7.

Tagawa, M., T. Shirasawa, S. Fujimura, and S. Sakiyama, Identification of a rat protein tyrosine phosphatase gene preferentially expressed in the embryonal brain. Cell Mol Biol Res, 1994. 40(7-8): p. 627-31.

Shirasawa, T., K. Sakamoto, and H. Tkahashi, Molecular cloning and evolutional analysis of a mammalian homologue of the Distal-less 3 (Dlx-3) homeobox gene. FEBS Lett, 1994. 351(3): p. 380-4.

Ohno, H., S. Goto, S. Taki, T. Shirasawa, H. Nakano, S. Miyatake, T. Aoe, Y. Ishida, H. Maeda, T. Shirai, and et al., Targeted disruption of the CD3 eta locus causes high lethality in mice: modulation of Oct-1 transcription on the opposite strand. EMBO J, 1994. 13(5): p. 1157-65.

Mori, H., K. Ishii, T. Tomiyama, Y. Furiya, N. Sahara, S. Asano, N. Endo, T. Shirasawa, and K. Takio, Racemization: its biological significance on neuropathogenesis of Alzheimer's disease. Tohoku J Exp Med, 1994. 174(3): p. 251-62.

Kitamura, M., T. Shirasawa, and N. Maruyama, Gene transfer of metalloproteinase transin induces aberrant behavior of cultured mesangial cells. Kidney Int, 1994. 45(6): p. 1580-6.

Kitamura, M., N. Maruyama, T. Shirasawa, R. Nagasawa, K. Watanabe, M. Tateno, and T. Yoshiki, Expression of an endogenous retroviral gene product in human placenta. Int J Cancer, 1994. 58(6): p. 836-40.

Asao, H., N. Tanaka, N. Ishii, M. Higuchi, T. Takeshita, M. Nakamura, T. Shirasawa, and K. Sugamura, Interleukin 2-induced activation of JAK3: possible involvement in signal transduction for c-myc induction and cell proliferation. FEBS Lett, 1994. 351(2): p. 201-6.

Akashi, T., T. Shirasawa, and K. Hirokawa, Gene expression of CD24 core polypeptide molecule in normal rat tissues and human tumor cell lines. Virchows Arch, 1994. 425(4): p. 399-406.

1993

Shirasawa, T., K. Ohnishi, S. Hagiwara, K. Shigemoto, Y. Takebe, K. Rajewsky, and T. Takemori, A novel gene product associated with mu chains in immature B cells. EMBO J, 1993. 12(5): p. 1827-34.

Shirasawa, T., T. Akashi, K. Sakamoto, H. Takahashi, N. Maruyama, and K. Hirokawa, Gene expression of CD24 core peptide molecule in developing brain and developing non-neural tissues. Dev Dyn, 1993. 198(1): p. 1-13.

Kitamura, M., T. Shirasawa, T. Mitarai, T. Muramatsu, and N. Maruyama, A retinoid responsive cytokine gene, MK, is preferentially expressed in the proximal tubules of the kidney and human tumor cell lines. Am J Pathol, 1993. 142(2): p. 425-31.

Kasai, M., T. Shirasawa, M. Kitamura, K. Ishido, E. Kominami, and K. Hirokawa, Proenzyme from of cathepsin L produced by thymic epithelial cells promotes proliferation of immature thymocytes in the presence of IL-1, IL-7, and anti-CD3 antibody. Cell Immunol, 1993. 150(1): p. 124-36.

Ikeda, T., T. Shirasawa, Y. Esaki, S. Yoshiki, and K. Hirokawa, Osteopontin mRNA is expressed by smooth muscle-derived foam cells in human atherosclerotic lesions of the aorta. J Clin Invest, 1993. 92(6): p. 2814-20.

1992

Usuda, S., T. Takemori, M. Matsuoka, T. Shirasawa, K. Yoshida, A. Mori, K. Ishizaka, and H. Sakano, Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism. EMBO J, 1992. 11(2): p. 611-8.

Tsunetsugu-Yokota, Y., T. Minekawa, K. Shigemoto, T. Shirasawa, and T. Takemori, Characterization of a new subgroup of human Ig V lambda cDNA clone and its expression. Mol Immunol, 1992. 29(6): p. 723-8.

Shirasawa, T., K. Sakamoto, T. Akashi, H. Takahashi, and A. Kawashima, Nucleotide sequence of rat elongation factor-1 alpha cDNA. Nucleic Acids Res, 1992. 20(4): p. 909.

Shirasawa, T., I. Miyazoe, S. Hagiwara, H. Kimoto, K. Shigemoto, M. Taniguchi, and T. Takemori, Heavy chain variable (VH) region diversity generated by VH gene replacement in the progeny of a single precursor cell transformed with a temperature-sensitive mutant of Abelson murine leukemia virus. J Exp Med, 1992. 176(4): p. 1209-14.

Kikuchi, N., H. Igari, T. Kawashima, I. Onozaki, and T. Shirasawa, [Diagnosis of pulmonary tuberculosis in general hospital "a study of 114 cases"]. Kekkaku, 1992. 67(7): p. 495-507.

Itoh, Y., N. Maruyama, M. Kitamura, T. Shirasawa, K. Shigemoto, and T. Koike, Induction of endogenous retroviral gene product (SU) as an acute-phase protein by IL-6 in murine hepatocytes. Clin Exp Immunol, 1992. 88(2): p. 356-9.

Fujita, T., T. Shirasawa, K. Uchida, and N. Maruyama, Isolation of cDNA clone encoding rat senescence marker protein-30 (SMP30) and its tissue distribution. Biochim Biophys Acta, 1992. 1132(3): p. 297-305.

Scientific Papers 1990 -

1990

Takemori, T., J. Mizuguchi, I. Miyazoe, M. Nakanishi, K. Shigemoto, H. Kimoto, T. Shirasawa, N. Maruyama, and M. Taniguchi, Two types of mu chain complexes are expressed during differentiation from pre-B to mature B cells. EMBO J, 1990. 9(8): p. 2493-500.

1989

Kimoto, H., T. Shirasawa, M. Taniguchi, and T. Takemori, B cell precursors are present in the thymus during early development. Eur J Immunol, 1989. 19(1): p. 97-104.

Kikuchi, N., I. Onozaki, and T. Shirasawa, [Community acquired pulmonary infections in a general hospital]. Nihon Kyobu Shikkan Gakkai Zasshi, 1989. 27(2): p. 166-72.

1987

Takemori, T., I. Miyazoe, T. Shirasawa, M. Taniguchi, and T. Graf, A temperature-sensitive mutant of Abelson murine leukemia virus confers inducibility of IgM expression to transformed lymphoid cells. EMBO J, 1987. 6(4): p. 951-6.